By L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)
Flow cytometry has speedily developed right into a strategy for quick research of DNA content material, mobile marker expression and digital sorting of cells of curiosity for extra investigations. stream cytometers are being widely used for tracking of mobile DNA content material, phenotype expression, drug delivery, calcium flux, proliferation and apoptosis. Phenotypic research of marker expression in leukemic cells has develop into a big instrument for diagnostic and healing tracking of sufferers. contemporary reviews have explored using movement cytometry for tracking hormone receptor expression in human strong tumors and for reports in human genomics. Contributions within the present quantity are in response to shows made on the First Indo-US workshop on movement Cytometry during which specialists from united states, united kingdom and India mentioned functions of move cytometry in organic and scientific examine. This ebook can be of curiosity to put up graduates and researchers within the fields of pathology, cytology, phone biology and molecular biology.
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Extra info for Advanced Flow Cytometry: Applications in Biological Research
369-377 (1990). Acknowledgements Collaborators at the National Flow Cytometry Resource, Los Alamos National Laboratory, who have made outstanding contributions to our development of flow cytogenetics include James Jett, Carolyn Bell, Joe Fawcett, John Martin, and Larry Deaven. This work was conducted under the auspices of the Department of Energy and with support from the National Institutes of Health, Division of Research Resources, Grant RRO 1315. Appendices Protocols for chromosome isolation and staining.
1 Jlg colcemidlml of tissue culture media for 12-15 hours. 35 2. Prepare fresh chromosome isolation buffer and place on ice. 3. Collect suspension cells in 50 ml centrifuge tubes, at 40°C. 4. Adjust supernatant volume to achieve a cell concentration of 8 x 106 mitotic cells per tube. 5. Centrifuge at 100 xg (approximately 800-1,000 rpm) for 8 min at 4 0c. 6. Aspirate supernatant, leaving as little as possible behind. Flick tube to loosen pellet. 7. Cell swelling. a. Add 5ml of Ohnuki's hypotonic swelling buffer per tube.
Laser scanning cytometry (LSC) allows detailed analysis of the cell cycle in PI stained human fibroblasts (TIG7). Cell Prolif 30 (3-4): 139-147. 6. Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schram! P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP (1998). Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4: 844-847. 7. Luther E, Kamentsky LA (1996). Resolution of mitotic cells using laser scanning cytometry. Cytometry 23: 272-278 8. Mait A, McKenna WG, Muschel RJ (1997).
Advanced Flow Cytometry: Applications in Biological Research by L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)